Biochem/physiol Actions

Cell permeable: no

EC50 = 510 pM in activating phospholipase C-mediated inositol phosphate formation in human U266 myeloma cells

Reversible: no

Product does not compete with ATP.

Primary TargetFormyl Peptide Receptor-Like 1

General description

A potent chemoattractant that enhances leukocyte activity via activation of Formyl Peptide Receptor-Like 1 (FPRL1). More poteut than fmlp at inducing neutrophil chemotaxis, neutrophil complement receptor 3. Shown to activate phospholipase C-mediated inositol phosphate formation in human U266 myeloma cells (EC₅₀ = 510 pM) and in U937 and HL60 cells. Has no effect inositide phosphate synthecs in a variety of other cell lines (e.g. NIH3T3, PC12, Daudi, Sp2, Jurkat, H9, Molt-4, SupT-1, K562, and RBL-2H3). Radioiodinatable at the tyrosine residue.

A potent chemoattractant that enhances leukocyte activity via activation of Formyl Peptide Receptor-Like 1 (FPRL1). Shown to activate phospholipase C-mediated inositol phosphate formation in human U266 myeloma cells (EC₅₀ = 510 pM). Also shown to stimulate inositol phosphate formation in U937 and HL60 cells, but has no effect on a variety of other cell lines (e.g. NIH3T3, PC12, Daudi, Sp2, Jurkat, H9, Molt-4, SupT-1, K562, and RBL-2H3). Stimulates phagocyte chemotaxis and [Ca²⁺] flux, and is more effective than fMLP (Cat. No. 05-22-2500) in generating superoxide in human neutrophils. Radioiodinatable at the tyrosine residue.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bae, Y.S., et al. 2004. J. Immunol.173, 607.Bae, Y.S., et al. 2003. Mol. Pharmacol.64, 841.Le, Y., et al. 1999. J. Immunol.163, 6777.Seo, J.K., et al. 1997. J. Immunol.158, 1895.Baek, S.H., et al. 1996. J. Biol. Chem.271, 8170.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

H-Trp-Lys-Tyr-Met-Val-D-Met-CONH₂

Warning

Toxicity: Standard Handling (A)